Journal: The Journal of Biological Chemistry

Article Title: Streptococcus pneumoniae Endopeptidase O (PepO) Is a Multifunctional Plasminogen- and Fibronectin-binding Protein, Facilitating Evasion of Innate Immunity and Invasion of Host Cells *

doi: 10.1074/jbc.M112.405530

Figure Lengend Snippet: Clinical S. pneumoniae isolates used in the present study

Article Snippet: C , representative flow cytometry data for the surface presentation of PepO on S. pneumoniae NTC10319 serotype 35A. table ft1 table-wrap mode="anchored" t5 caption a7 Bacterial species Gene ID Gene sequence identity % S. pneumoniae R6 spr1491 100 S. pneumoniae P1031 SPP_1666 99 S. pneumoniae Hungary 19A-6 SPH_1756 99 S. pneumoniae JJA SPJ_1542 99 S. pneumoniae TIGR4 SP_1647 99 S. pneumoniae G54 SPG_1557 99 S. pneumoniae 70585 SP70585_1688 99 S. pneumoniae ST556 MYY_1572 99 S. pneumoniae TCH8431/19A HMPREF0837_11890 99 S. pneumoniae CGSP14 SPCG_1620 99 S. pneumoniae 670–6B SP670_1734 99 S. pneumoniae ATCC 700669 SPN23F16490 99 S. pneumoniae gamPNI0373 HMPREF1038_01631 99 S. pneumoniae OXC141 SPNOXC_14460 99 S. pneumoniae AP200 SPAP_1655 99 S. pneumoniae INV104 INV104_13990 99 S. pneumoniae INV200 SPNINV200_14720 99 S. pneumoniae Taiwan19F-14 SPT_1587 99 S. pneumoniae SPNA45 SPNA45_00593 99 S. pseudopneumoniae IS7493 SPPN_08030 97 Streptococcus mitis B6 smi_0639 93 Streptococcus oralis Uo5 SOR_1505 85 Streptococcus gordonii Challis NCTC7868 SGO_1799 72 Streptococcus suis 05ZYH33 SSU05_2082 70 S. suis 98HAH33 SSU98_2085 70 Streptococcus thermophilus CNRZ1066 str1885 68 S. thermophilus LMG18311 stu1885 68 S. thermophilus LMD-9 STER_1860 68 Streptococcus salivarius SK126 STRSA0001_0505 68 Streptococcus mutans UA159 SMU_2036 67 Streptococcus pyogenes MGAS2096 MGAS2096_Spy1815 66 S. pyogenes MGAS9429 MGAS9429_Spy1793 66 S. pyogenes MGAS10394 M6_Spy1781 66 S. pyogenes MGAS8232 spyM18_2153 66 Open in a separate window pepO gene sequence identity in pneumococci and other bacterial species

Techniques:

Journal: The Journal of Biological Chemistry

Article Title: Streptococcus pneumoniae Endopeptidase O (PepO) Is a Multifunctional Plasminogen- and Fibronectin-binding Protein, Facilitating Evasion of Innate Immunity and Invasion of Host Cells *

doi: 10.1074/jbc.M112.405530

Figure Lengend Snippet: Distribution of pepO gene and its expression in various pneumococcal isolates. A, agarose gel electrophoresis image showing the amplified product of the pepO gene (1893 bp) in clinical isolates. B, immunoblots demonstrating the presence of PepO in the bacterial whole cell lysates of pneumococcal isolates. The molecular weight protein marker was used as a reference. C, representative flow cytometry data for the surface presentation of PepO on S. pneumoniae NTC10319 serotype 35A.

Article Snippet: C , representative flow cytometry data for the surface presentation of PepO on S. pneumoniae NTC10319 serotype 35A. table ft1 table-wrap mode="anchored" t5 caption a7 Bacterial species Gene ID Gene sequence identity % S. pneumoniae R6 spr1491 100 S. pneumoniae P1031 SPP_1666 99 S. pneumoniae Hungary 19A-6 SPH_1756 99 S. pneumoniae JJA SPJ_1542 99 S. pneumoniae TIGR4 SP_1647 99 S. pneumoniae G54 SPG_1557 99 S. pneumoniae 70585 SP70585_1688 99 S. pneumoniae ST556 MYY_1572 99 S. pneumoniae TCH8431/19A HMPREF0837_11890 99 S. pneumoniae CGSP14 SPCG_1620 99 S. pneumoniae 670–6B SP670_1734 99 S. pneumoniae ATCC 700669 SPN23F16490 99 S. pneumoniae gamPNI0373 HMPREF1038_01631 99 S. pneumoniae OXC141 SPNOXC_14460 99 S. pneumoniae AP200 SPAP_1655 99 S. pneumoniae INV104 INV104_13990 99 S. pneumoniae INV200 SPNINV200_14720 99 S. pneumoniae Taiwan19F-14 SPT_1587 99 S. pneumoniae SPNA45 SPNA45_00593 99 S. pseudopneumoniae IS7493 SPPN_08030 97 Streptococcus mitis B6 smi_0639 93 Streptococcus oralis Uo5 SOR_1505 85 Streptococcus gordonii Challis NCTC7868 SGO_1799 72 Streptococcus suis 05ZYH33 SSU05_2082 70 S. suis 98HAH33 SSU98_2085 70 Streptococcus thermophilus CNRZ1066 str1885 68 S. thermophilus LMG18311 stu1885 68 S. thermophilus LMD-9 STER_1860 68 Streptococcus salivarius SK126 STRSA0001_0505 68 Streptococcus mutans UA159 SMU_2036 67 Streptococcus pyogenes MGAS2096 MGAS2096_Spy1815 66 S. pyogenes MGAS9429 MGAS9429_Spy1793 66 S. pyogenes MGAS10394 M6_Spy1781 66 S. pyogenes MGAS8232 spyM18_2153 66 Open in a separate window pepO gene sequence identity in pneumococci and other bacterial species

Techniques: Expressing, Agarose Gel Electrophoresis, Amplification, Western Blot, Molecular Weight, Marker, Flow Cytometry

Journal: The Journal of Biological Chemistry

Article Title: Streptococcus pneumoniae Endopeptidase O (PepO) Is a Multifunctional Plasminogen- and Fibronectin-binding Protein, Facilitating Evasion of Innate Immunity and Invasion of Host Cells *

doi: 10.1074/jbc.M112.405530

Figure Lengend Snippet: Secretion of PepO by pneumococci. A, immunoblot showing the presence of PepO in the culture supernatant of various isolates of S. pneumoniae. Mid-logarithmic phase culture supernatant of pneumococcal isolates were collected and subjected to SDS-PAGE. Presence of PepO protein in the culture supernatant was analyzed using rabbit anti-PepO polyclonal Abs. B and C, growth phase-dependent secretion of PepO by S. pneumoniae strain NCTC10319 serotype 35A. Bacteria were cultured in THY media and the culture supernatant was collected at regular intervals (B) and separated by 10% SDS-PAGE (C). After blotting, the PepO protein was detected using rabbit anti-PepO polyclonal Abs. THY media containing no bacteria was used as control and the molecular weight protein marker was used as a reference.

Article Snippet: C , representative flow cytometry data for the surface presentation of PepO on S. pneumoniae NTC10319 serotype 35A. table ft1 table-wrap mode="anchored" t5 caption a7 Bacterial species Gene ID Gene sequence identity % S. pneumoniae R6 spr1491 100 S. pneumoniae P1031 SPP_1666 99 S. pneumoniae Hungary 19A-6 SPH_1756 99 S. pneumoniae JJA SPJ_1542 99 S. pneumoniae TIGR4 SP_1647 99 S. pneumoniae G54 SPG_1557 99 S. pneumoniae 70585 SP70585_1688 99 S. pneumoniae ST556 MYY_1572 99 S. pneumoniae TCH8431/19A HMPREF0837_11890 99 S. pneumoniae CGSP14 SPCG_1620 99 S. pneumoniae 670–6B SP670_1734 99 S. pneumoniae ATCC 700669 SPN23F16490 99 S. pneumoniae gamPNI0373 HMPREF1038_01631 99 S. pneumoniae OXC141 SPNOXC_14460 99 S. pneumoniae AP200 SPAP_1655 99 S. pneumoniae INV104 INV104_13990 99 S. pneumoniae INV200 SPNINV200_14720 99 S. pneumoniae Taiwan19F-14 SPT_1587 99 S. pneumoniae SPNA45 SPNA45_00593 99 S. pseudopneumoniae IS7493 SPPN_08030 97 Streptococcus mitis B6 smi_0639 93 Streptococcus oralis Uo5 SOR_1505 85 Streptococcus gordonii Challis NCTC7868 SGO_1799 72 Streptococcus suis 05ZYH33 SSU05_2082 70 S. suis 98HAH33 SSU98_2085 70 Streptococcus thermophilus CNRZ1066 str1885 68 S. thermophilus LMG18311 stu1885 68 S. thermophilus LMD-9 STER_1860 68 Streptococcus salivarius SK126 STRSA0001_0505 68 Streptococcus mutans UA159 SMU_2036 67 Streptococcus pyogenes MGAS2096 MGAS2096_Spy1815 66 S. pyogenes MGAS9429 MGAS9429_Spy1793 66 S. pyogenes MGAS10394 M6_Spy1781 66 S. pyogenes MGAS8232 spyM18_2153 66 Open in a separate window pepO gene sequence identity in pneumococci and other bacterial species

Techniques: Western Blot, SDS Page, Bacteria, Cell Culture, Control, Molecular Weight, Marker

Journal:

Article Title: Nomenclature of Major Antimicrobial-Resistant Clones of Streptococcus pneumoniae Defined by the Pneumococcal Molecular Epidemiology Network

doi: 10.1128/JCM.39.7.2565-2571.2001

Figure Lengend Snippet: Properties of 16 pneumococcal clones a

Article Snippet: ATCC accession numbers for the 16 clones are given in Table . table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Clone Reference strain (ATCC accession no.) Serotype MIC (μg/ml) of: Reference(s) PEN CTX ERY CLI CHL TET SXT Spain 23F -1 SP264 (ATCC 700669) 23F 2 0.5 0.06 0.06 16 32 4/76 7, 32, 37 Spain 6B -2 GM17 (ATCC 700670) 6B 2 1 0.12 0.12 16 32 4/76 38 Spain 9V -3 TL7/1993 (ATCC 700671) 9V 2 1 0.12 0.12 2 ≤0.5 8/152 7, 29 Tennessee 23F -4 CS111 (ATCC 51916) 23F 0.12 32 32 0.12 2 ≤0.5 4/76 33, 49 Spain 14 -5 MS22 (ATCC 700902) 14 2 1 0.06 0.12 16 32 2/38 8 Hungary 19A -6 HUN663 (ATCC 700673) 19A 2 0.5 >32 >32 16 16 2/38 31, 38 South Africa 19A -7 17619 (ATCC 700674) 19A 0.5 0.5 0.25 0.25 2 ≤0.5 4/152 51 South Africa 6B -8 50803 (ATCC 700675) 6B 0.5 0.25 0.12 0.12 2 ≤0.5 1/19 51 England 14 -9 PN93/872/B (ATCC 700676) 14 0.03 0.03 32 0.12 2 ≤0.5 0.12/4.2 17 CSR 14 -10 87-029055 (ATCC 700677) 14 8 1 >32 >32 16 32 0.25/4.8 16, 19 CSR 19A -11 91-006571 (ATCC 700678) 19A 4 1 >32 >32 32 32 4/76 16, 19 Finland 6B -12 43362 Fi10 (ATCC 700903) 6B 1 >32 >32 4 32 8/152 48 South Africa 19A -13 51702 (ATCC 700904) 19A 8 2 >8 >8 32 8 8/152 51 Taiwan 19F -14 TW31 (ATCC 700905) 19F 2 1 4 0.12 2 16 4/76 47 Taiwan 23F -15 TW17 (ATCC 700906) 23F 1–2 1 >32 >32 2 16 0.5/9.5 47 Poland 23F -16 178 23F 8 8 >8 0.25 16 16 4/76 41 Open in a separate window a PEN, penicillin; CTX, cefotaxime; ERY, erythromycin; CLI, clindamycin; CHL, chloramphenicol; TET, tetracycline; SXT, trimethoprim-sulfamethoxazole; CSR, Czech Republic.

Techniques: Clone Assay